The method consists in drying at defined
pressure and temperature until the sample attains constant mass.
In the present study the soluble solids content was determined
using a digital Pocket PAL-1 refractometer made by ATAGO (Japan).
The measuring range of the apparatus was between 0 and 53 Brix
degrees with automatic temperature compensation ranging from
10 to 75C. Pumpkin puree reaction was determined with a pHmeter PX-processor PM-600 made by TMS Electronics. Sugars were
assayed according to the Lane–Eynon method