P. alata has an increasing economic importance worldwide.
However, few studies aiming at the establishment of in vitro systems
as a toolfor propagation and conservation have been reported,
and efficient culture protocols have not been achieved so far. These
studies are of great interest, mainly when we consider seed behavior
and difficulties already reported during in vitro establishment
of this species (Catunda et al., 2003). In this work, we developed
efficient methods for in vitro plant production of P. alata, as well as
for induction of callogenesis and establishment of cell suspension
cultures