Fig. 2. A physically crosslinked hydrogel formed upon mixing of two modularly designed polypeptides for the encapsulation of mouse adipose-derived stem cells (mASCs).
(a) Schematic of the MITCH network (left) formed after mixing of individual components (right). Protein sequences are shown using single-letter amino acid abbreviations.
(b) Images of mASC (Fluc+) transplant sites in nude mice; sites are demarcated with dotted lines (day 0, left) and with bioluminescence (BLI) total flux overlay (day 3, right).
(c) BLI measurements of cell retention. Data normalized to day 1 and reported as mean ± SEM; n = 5 or 6; ⁄p < 0.0001. Adapted from Ref. [100], copyright 2012, reprinted with
permission from Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.