Ten grams of each sample (untreated and treated) was obtained
aseptically and homogenized with 90 mL chilled maximal recovery
diluent (Oxoid, Basingstoke, Hampshire, England) in a filter stomacher
bag with a Stomacher 400 Circulator (Seward Laboratory, London, UK)
at 240 rpm for 2 min. Further decimal dilutions were performed with
the same diluent and duplicates of at least three appropriate dilutions
were plated on appropriate media.