Test inhibitory of Staphylococcus aureus and Staphylococcus epidermidis by Agar well diffusion method
3.3.1 using Chitosan solution on the inhibition of bacteria by using cotton swab dip in MHB that have bacterial after that streak bacteria in MHA about 3 way for the bacteria spread in plate. (prepare the bacteria 20 min)
3.3.2 use cork borer No.3 drilling on plate food labels, and then use loop remove residual agar pieces3.3.3 drop Chitosan solution at concentration 0.5 0.25 0.125 and 1.0 percent in each hole 80 μl 3.3.4 drops Tetracycline into the hole to determine that the positive control and drop distilled water into the hole to determine that negative control for compare inhibitory efficiency with Chitosan solution. 3.3.5 after that ferment at 37° c for 16-18 hours
3.3.6 and finally measure the diameter of clear zone for calculation percentage of inhibition compared with antibiotic Tetracycline