2.2.1. Antitumor activity (cytotoxic activity)
A preliminary screening for cytotoxic activity towards Ehrlich ascities carcinoma using a set
of sterile test tubes; where 2.5 ×105 tumor cells /ml were suspended in phosphate buffer saline. 25, 50,
100 µg /ml of the extract were added to the suspension, kept at 37º C for 4 hrs. Trypan blue dye
exclusion test was carried out to calculate the percentage of non viable cells [11].