The solution was then distilled with sodium hydroxide (added in small quantity) which converts ammonium salt into ammonia. The ammonia was trapped using 1% (v/v) boric acid and titrated using 0.1 N HCl solution until end point was reached. Both distillation and titration and with subse-quent determination of nitrogen and protein content were automatically done by the Microkjeldahl apparatus. Pro-tein contents recorded are averages of three determination