The PCR amplification
was carried out in a 20 l reaction mixture
containing GoTaq® Green Master Mix (Promega,
USA), 100 ng of DNA and 0.5 M of each primer.
The PCR profile was initially at 95 oC for 3 min,
followed by 35 cycles at 95 oC for 1 min, 68 oC for 1
min and 72 oC for 1.5 min and 5 min at 72 oC for
final extension. The expected PCR products of 1,356
bp were analyzed on a 1 % (w/v) agarose gel by
electrophoresis.