Molecular biology techniques, including plasmid transformation of Escherichia coli DH5(F'Phi80dlacZ DeltaM15 Delta(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK-mKþ)phoA supE44 lambda-thi-1), bacterial cultivation, antibiotic selection, and agarose gel electrophoresis were carried out as described 6)or by following the manufacturer’sinstructions.