The basic material for analyses comprised mechanically recovered
meat from carcasses of broiler chickens with removed breast
muscles and legs, obtained with the use of an RM 500 device by
Lima (Lima S.A.S.; Quimper, France). The meat material was purchased
in a local poultry abattoir and processing plant. Immediately
after separation the temperature of MRPM did not exceed
+6 C. The method to obtain the myofibril preparation (MP) was
adopted following the procedure described in the patent claim
(Kijowski et al., 1996). The meat material was washed
(0.169 mol/L) with 1% aqueous solution of sodium chloride and
next with water (MDOM: water; 1:3; w/v), followed by separation
of fat and connective tissue using sieves. MP samples were modified
by the addition of 3 g/kg enzymatic preparation for 5 h at 6–
7 C.
The enzymatic modification of MP was performed using a commercial
ACTIVA WM preparation by Ajinomoto Co. Ltd. (Barentz,
Poland). The preparation contained 1% microbial transglutaminase
(MTG) (Streptoverticillium sp.) with the activity of 100 U/g preparation
and 99% maltodextrin serving the role of the enzyme carrier
(Ajinomoto’s specifications). One unit was the amount of the
enzyme which catalysed the formation of 1 lmol of hydroxamic
acid/min at 37 C.