This project aims to study the oil biodiesel from vegetable oil to customize and use in the single-cylinder gasoline engine. The study on production of biodiesel oil from vegetable oil and the effect voltage quantity from the spark plug as oil biodiesel with ethanol from the comparison, the value of efficiency bio-diesel with its ethanol fuel usage with 91, starting from Apply vegetable oil .It's already coming through plant chemicals, called Transesterification for the production of biodiesel, and then it blended with ethanol purity 95% to determine the appropriate rate to be applied to a single-cylinder gasoline engine. From the experimental results, it was found that the proper ratio is the ratio of fuel to it 10% biodiesel mixed with ethanol purity 95% ratio 90% when the ratio test to measure pollution trial results showed that the value of the pollution emissions of HC and CO gasohol 91 is high than biodiesel blending with ethanol among the test condition 2 best pollution emissions of Nox its biodiesel blending ethanol is higher than the rate of gasohol 91 fuel and waste oil 91 of its biodiesel blending ethanol with less fuel consumption rate of gasohol 91 fuel.