Volatile compounds in separated fractions of cocoa pulp and nibs
were extracted by head space–solid phase microextraction (HS–SPME)
using a 50/30 μm divinylbenzene/carboxene/polydimethylsiloxane
fiber (Supelco, Bellefonte, PA, USA) and analyzed by an Agilent 5975
gas chromatography–mass spectrometer (GC–MS) equipped with a
Hewlett Packard capillary column (HP-5MS, 30 m length × 0.25 mm
ID × 0.25 μm thickness). The conditions of extraction and GC–MS as
well as the method for identification of volatile compounds were the
same as described by Ho et al. (2014). The volatile compounds in each
sample were extracted and analyzed in duplicate and the results are
expressed as an average.