The lateral flow assay for the qualitative detection of S. pneumonia and Legionella pneumophila serogroup 1 antigen in urine from SSI Diagnostica (Hillerød, Denmark)
was performed according to the instruction of the manufacturer.
Briefly, three drops of patient urine and two drops of running buffer were gently mixed in a test tube. The test strip was inserted. Test results were read after 15 min incubation, interpreted by the presence or absence of visually detectable pink (S. pneumoniae) or blue (L. pneumophila) coloured lines