The surface sterilised explants were trimmed and placed on Murashige and Skoog
(MS) medium, Gamborgs B5 and Woody Plant Medium (WPM) supplemented with 3% sucrose and different concentrations of 6-benzylaminopurine (BAP) and kinetin, adjusted to the pH of 5.7 ± 0.1 prior to the addition of gelling agent and sterilized in autoclave at 15 lbs. pressure for 20 minutes (temp. approx. 121 ºC).