which have been reported. Although Spectrophotometer
is a fast and simple method it is not possible to
determine caffeine directly in coffee beans by conventional
UV absorption measurement due to the spectral overlap
(Zhang et al., 2005). On the other hand, the derivative spectrophotometer
is relatively easy; however, it is not reliable
for the determination of small concentration of caffeine in
samples. With HPLC methods the use of expensive equipments
and the demand for more operator attention prevent