Cloning; using TA cloning system which is designed for direct cloning of PCR products to the TA cloning vector. PCR amplification products generated using non-proofreading thermostable DNA polymerases, such as Taq DNA polymerase, that leave single 3’-dA overhangs on their reaction products. The TA cloning system vector contains single 3’-dT overhangs that are compatible with direct ligation of these products without the need for intermediate reactions