Nodal explants containing lateral buds of actively field-grown ‘Perfume Delight’ rose were used for multiplication experiments. They were cut in 3-4 cm length segments and surface-disinfested using 70% ethanol for 10-20 s and
then immersed in 40% (v/v) Clorox TM solution of commercial laundry bleach (5.25% NaOCl) containing 2 drops of Tween-20 emulsifier to aid wetting for 20 min. The sterilized explants were washed 2-3 times with sterile distilled water to remove disinfecting solution. They were trimmed down to 1 cm long prior to transferring to shoot multiplication medium.