Biochemical measurement
Subjects were requested to refrain from exercise for 24
hours and to fast for at least 10 hours before blood was
drawn in the morning. Blood samples were collected on
Buttock, hip, waist, and back
Shoulders
the day of the initiation of the massage or control
period, and on the day following each massage or control
period. All samples were stored at −80 Celsius and
analyzed in batch at the end of the study. Serum Cterminal
telopeptide of type I collagen (CTx-I), total
procollagen type 1 amino-terminal propeptide (P1NP),
N-MID osteocalcin, and insulin were determined by
electrochemiluminescence immunoassay on a Cobas e
411 analyzer (Roche Diagnostics, Mannheim, Germany).
The intra-assay precision was 3.8%, 3.8%, 1.4% and
1.9%, respectively
Biochemical measurementSubjects were requested to refrain from exercise for 24hours and to fast for at least 10 hours before blood wasdrawn in the morning. Blood samples were collected onButtock, hip, waist, and backShouldersthe day of the initiation of the massage or controlperiod, and on the day following each massage or controlperiod. All samples were stored at −80 Celsius andanalyzed in batch at the end of the study. Serum Cterminaltelopeptide of type I collagen (CTx-I), totalprocollagen type 1 amino-terminal propeptide (P1NP),N-MID osteocalcin, and insulin were determined byelectrochemiluminescence immunoassay on a Cobas e411 analyzer (Roche Diagnostics, Mannheim, Germany).The intra-assay precision was 3.8%, 3.8%, 1.4% and1.9%, respectively
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