The remaining four sera showed weak immunoreactivity with the proteins 30S ribosomal protein S1 (FTT_0183c), 50S ribosomal protein L7/L12 (FTT_0143) and hypothetical protein (FTT_1778c).
In addition, a single serum sample (#208) was screened against antigen separated in the basic pH range (Fig. 2c).
Three weakly immunoreactive regions were aligned with protein spot trains on equivalent 2D-PAGE and identified as peptidase, M24 family protein (FTT_0609), malate dehydrogenase (FTT_0535c) and oxidoreductase, short-chain dehydrogenase family protein (FTT1201c) (Fig. 2e).