Moreover, the combined effect of growth temperature and pH in the induction of hyphae and pseudohyphae has also been observed previously [13]. The optima for fila-mentation in C. albicans are pH 7 and 37°C temperature. However, increase in either parameters between these optima or parameter limits result in significantly low or no filamentation [37]. When cells are released into fresh media at 37°C but at pH 7, the cells are synchronously evaginated to form germ tube. These cell cultures con-tinue to grow as mycelia form as long as pH is 7 and temperature is 37°C.