Data collection and statistical analysis of the comet assay results
For quantitative analysis of nuclear DNA damage HepG2 cells, the slides were viewed at 200× magnification with an epifluorescence microscope (Olympus BX 50) using a BP 515–560 nm filter and BA 590 nm barrier filter. Microscopic images of comets were captured by a digital camera (Hammamatsu Orca
2) connected to a computer, and the comets were scored