In summary, a multiplex PCR-based approach in combination
with an enrichment strategy, has been used for sensitive
detection of E. coli O157:H7 in a variety of contrasting
environmental materials. Speed and sensitivity make this
assay potentially useful for screening rural drinking water
supplies that are currently at high risk of E. coli O157:H7
contamination. Employing the conditions reported in this
study, it is possible to obtain sensitive detection of E. coli
O157:H7 in soil and water within one working day.