This study emphasized the importance of
colchicine treatment for mutation induction both
on directly dropping on inflorescences and seed
soaking. Although some characteristics such as
stomatal density and guard cell size were introduced
for determining the ploidy chromosome set, but in
this study, only guard cell size (width and length)
showed the value decreasing when colchicine
concentration was increased. For the study of DNA
content by the flow cytometry, except to normal
diploidy (2x), many types of mixoploid occurred in
both experiments. Directly treated the inflorescences
with colchicine showed mixoploidy of trisomic
(2x + 1) and tetrasomic (2x + 2) with disomic.
Soaking seeds with colchicine showed many types
of mixoploid with disomic (2x) such as trisomic,
tetrasomic, pentasomic (2x + 3) and hexasomic
(2x + 4). Extra chromosome number of incomplete
aneuploidy and the complex of mixoploid increased
when the higher concentration of colchicine was
treated.
This study emphasized the importance of
colchicine treatment for mutation induction both
on directly dropping on inflorescences and seed
soaking. Although some characteristics such as
stomatal density and guard cell size were introduced
for determining the ploidy chromosome set, but in
this study, only guard cell size (width and length)
showed the value decreasing when colchicine
concentration was increased. For the study of DNA
content by the flow cytometry, except to normal
diploidy (2x), many types of mixoploid occurred in
both experiments. Directly treated the inflorescences
with colchicine showed mixoploidy of trisomic
(2x + 1) and tetrasomic (2x + 2) with disomic.
Soaking seeds with colchicine showed many types
of mixoploid with disomic (2x) such as trisomic,
tetrasomic, pentasomic (2x + 3) and hexasomic
(2x + 4). Extra chromosome number of incomplete
aneuploidy and the complex of mixoploid increased
when the higher concentration of colchicine was
treated.
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