Culture conditions and statistical analysis
MS medium was used throughout the experiment. The pH
of the medium was adjusted to 5.8 using 0.1N NaOH and
0.1N HCl prior to adding 0.8 % (w/v) agar and the tubes
were autoclaved at 120 C and 105 kPa for 15 min. The
cultures were maintained under a 16/8-h light/dark photoperiod,
temperature being 22 ± 2 C. A light intensity
of 40 mol m-2s-1 was provided by cool white fluorescent
tubes (Philips, India). For callus induction at least 24
cultures with one explant each were used and observations
like percent response, callus growth and fresh weight were
made 45 days after culture. For callus regeneration with
various treatments at least 24 cultures each containing one
gram calli were raised and percent response and number
of embryos per gram calli were made 45 days after culture.
All the experiments were repeated three times.
Analysis of variance and Duncan’s multiple range test
were used for comparison among treatment means (Duncan
1955).
Plant Cell Tiss Organ Cult (2012) 110:141–151 143