However, the enzyme was not released when immobilized under a concentration of buffer of 5 mM and incubated at 25 mM of the corresponding buffer at all the studied pH values. Therefore, after the enzyme-support multi-ion exchange, the ionic strength that may prevent the immobilization is not enough to produce enzyme desorption from the MANAE support. All enzymes could be released to the medium using 150 mM of buffer, and the support could be reused in several adsorption/desorption cycles without any detrimental effect on its performance.