inaccessible due to the proper structure of the protein. A denaturation pre-treatment may facilitate subsequent enzymatic attack [3]. Different processes are described in Ref. [4] to obtain protein hydrolysates from cattle hide waste, which are mainly chemical processes based on calcium hydroxide. Hide waste can also be hydrolyzed by means of enzymatic hydrolysis with alkaline protease [5,6]. Enzymatic process is preferred over chemical process as it is more environment friendly. Studies are also carried out on an aqueous extraction of animal skin tissue followed by an
enzyme treatment at 40e70 C at various pHs [7].