30 Example 7. Conventional green tea and black tea prepared from the source identical
to ส่วนแยก ซีรัม of Camellia sinensis (i.e. Recentia® CS), have no inhibition of IL-1a induced by เอธอกซิเลตเตด alcohol.
Recentia® CS was compared to conventional green tea and black tea preparations 35 obtained from the same source for mitigating เอธอกซิเลตเตด alcohol -induced secretion of the
inflammatory ไซโตไคน์ IL-1 a.
Conventional green tea and conventional black tea preparations were obtained as described in Example 6. In particular, the preparations used for Example 6 and Example 7 are aliquots of identical material.
5 The แบบวิธี for the growth of HEK are described under Example 1. Treatment was
conducted by incubation of HEK with เอธอกซิเลตเตด alcohol (500 µg/ml for การชักนำ of IL-1a, as determined in Example 3) for 16 hours in presence of a range of concentrations of
Recentia® CS, or conventional green tea preparation or conventional black tea preparation.
10
As shown in the Figure 8, Recentia® CS ในรูปแบบ ที่ขึ้นอยู่กับ ขนาดยา inhibited เอธอกซิเลตเตด
alcohol-induced release of IL-1 a with estimated 1050 of about 0.70% (with notable inhibition
starting at the concentration of about 0.3%). However, neither green tea nor black tea at the
same concentrations showed notable inhibition. While the highest concentration of
15 Recentia® CS tested was 1%, this should not be construed as the high limit of concentration
range effective for inhibition. The data indicate that Recentia® CS, processed by patented
Zeta ส่วนแยก TM technology, has unique and diverse composition of natural constituents that
exhibit superior multifunctional benefits than preparations of green tea and black tea.
20 In Figure 8, "Baseline" refers to the concentration of IL-1a in ส่วนลอย of untreated
HEK. "การชักนำ" refers to the concentration of IL-1 a in ส่วนลอย of HEK treated with 500 µg/ml of เอธอกซิเลตเตด alcohol, which is the การชักนำ dose determined in Example 3. Due to variations inherent in biological test systems such as HEK cultures produced from cells
harvested from different donors or different donor body locations, response of HEK as
25 shown by concentrations of ไซโตไคน์ (e.g. IL-1a) and คีโมไคน์s (e.g. IL-8) may vary
between experiments, particularly the "Baseline" and "การชักนำ" concentrations.
Example 8. ส่วนแยก ซีรัม of Citrus limon (Recentia® CL) and Trifolium pratense
30 (Recentia® TP) inhibit SDS-induced IL-1a ในรูปแบบ ที่ขึ้นอยู่กับ ขนาดยา.
Citrus limon (Recentia® CL) and Trifolium pratense (Recentia® TP) are processed
as described in, for example, US patents 7473435, 8043635, and 8318220. They deliver
multiple benefits for free-radical scavenging properties, anti-inflammatory benefits, and photo 35 stabilization activity. Therefore, we evaluated Citrus limon (Recentia® CL) and Trifolium
pratense (Recentia® TP) for mitigating SDS-induced secretion of the inflammatory ไซโตไคน์
IL-1a.
The แบบวิธี for the growth of HEK are described under Example 1. Treatment was conducted by incubation of HEK with SDS alcohol (25 µg/mI for การชักนำ of IL-1a, as
determined in Example 1) for 16 hours in presence of a range of concentrations of
5 Recentia® CL, or Recentia® TP.
As shown in the Figure 9, Citrus limon (Recentia® CL) ในรูปแบบ ที่ขึ้นอยู่กับ ขนาดยา inhibited
IL-1a การปลดปล่อย ที่ชักนำ โดย SDS in HEK, with estimated 1050 of about 0.46% (with notable
inhibition starting at about 0.2%). Trifolium pratense (Recentia® TP) also ในรูปแบบ ที่ขึ้นอยู่กับ ขนาดยา 10 mitigated การปลดปล่อย ที่ชักนำ โดย SDS of the inflammatory ไซโตไคน์ IL-1 a, with 1% inhibiting about
35% (with notable inhibition starting at about 0.75%) of การปลดปล่อย ที่ชักนำ โดย SDS of IL-1a. While
the highest concentration of Recentia® CL and Recentia® TP tested was 1%, this should
not be construed as the high limit of concentration ranges effective for inhibition.
15 In Figure 9, "Baseline" refers to the concentration of IL-1a in ส่วนลอย of untreated
HEK. "การชักนำ" refers to the concentration of IL-1a in ส่วนลอย of HEK treated with 25
fag/ml of SDS, which is the การชักนำ dose determined in Example 1. Due to variations
inherent in biological test systems such as HEK cultures produced from cells harvested from
different donors or different donor body locations, response of HEK as shown by
20 concentrations of ไซโตไคน์ (e.g. IL-1a) and คีโมไคน์s (e.g. IL-8) may vary between
experiments, particularly the "Baseline" and "การชักนำ" concentrations.
In conclusion, the unique combination of surfactants with Zeta ส่วนแยก TM technology
derived natural ส่วนแยก ที่รวมถึง, without limitation, Camellia sinensis (Recentia® CS),
25 Citrus limon (Recentia® CL), and Trifolium pratense (Recentia® TP), provides a novel
approach to mitigate surfactant-induced skin irritation and inflammation responses and
impart the mildness to the next generation of surfactant-containing products.