.
However, average viable cell counts of P. marginalis 667
were higher than ten times the calculated LSD value in comparison
with the presumed viable load of this strain in electrolyzed NaCl
solution. The prolongation of the exposure time (5 min) did not
show further improvement (data not shown). Based on these results
the second in vitro assay was carried out using NEW produced
by chlorinated salt KCl at chlorine concentration between 100 and
400 mg/L, preferring MPN technique to plating method as a more
sensitive microbiological method. In order to reach these concentration
of free chlorine KCl was preferred to NaCl to prevent sodium
ions accumulation in water that could damage vegetable tissues
(Ritenour and Crisosto, 1996). For 12 Pseudomonas strains, untreated
cellular suspensions showed growth level above
11,000 MPN/ml whereas NEW treated Pseudomonas cell suspensions
(100 mg/L of free chlorine) were sufficient to reduce microbial
viability lower than 30 MPN/ml; to reach the same antimicrobial
activity the free chlorine concentration had to be increased to
200 mg/L for P. fluorescens L1A and I3B strains. P. aeruginosa
DSM939 was sensitive to contact with NEW containing 400, 200
and 100 mg/L of free chlorine. For all Pseudomonas bacteria, no
residual cell viability was recorded after additional 24 h of incubation
at 30 C. Since washing water used in processing plant is
contaminated by organic matter (from soil and vegetable tissues)
and by different microorganisms, based on results related to
.However, average viable cell counts of P. marginalis 667were higher than ten times the calculated LSD value in comparisonwith the presumed viable load of this strain in electrolyzed NaClsolution. The prolongation of the exposure time (5 min) did notshow further improvement (data not shown). Based on these resultsthe second in vitro assay was carried out using NEW producedby chlorinated salt KCl at chlorine concentration between 100 and400 mg/L, preferring MPN technique to plating method as a moresensitive microbiological method. In order to reach these concentrationof free chlorine KCl was preferred to NaCl to prevent sodiumions accumulation in water that could damage vegetable tissues(Ritenour and Crisosto, 1996). For 12 Pseudomonas strains, untreatedcellular suspensions showed growth level above11,000 MPN/ml whereas NEW treated Pseudomonas cell suspensions(100 mg/L of free chlorine) were sufficient to reduce microbialviability lower than 30 MPN/ml; to reach the same antimicrobialactivity the free chlorine concentration had to be increased to200 mg/L for P. fluorescens L1A and I3B strains. P. aeruginosaDSM939 was sensitive to contact with NEW containing 400, 200and 100 mg/L of free chlorine. For all Pseudomonas bacteria, noresidual cell viability was recorded after additional 24 h of incubationat 30 C. Since washing water used in processing plant iscontaminated by organic matter (from soil and vegetable tissues)และจุลินทรีย์ต่าง ๆ โดยอิงตามผลที่เกี่ยวข้องกับ
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