2.7. Statistical test
Two-way ANOVA was used to test if heating procedure and
vitamin D compound influenced retention in eggs (vitD3 and
25OHD3) and in bread (vitD3 and vitD2). One-way ANOVA was used
to test the retention of vitamin D3 in margarine as the only ingredient
and as ingredient in cake.
Least Significance Difference was used to test for differences between
the heating procedures, while t-tests were used to test, if the
retention differed from 100% (Excel 2010). P-value at minimum
0.05 was classified as a significant difference. All results are given
as average ± sd.