Excess organic extractant is needed for microalgae lutein extraction since lutein is bound with LHCs. Supercritical fluid extraction (SFE) using CO2 should not be efficient for microalgae lutein extraction since the polarity of free lutein is high. Polar cosolvents including methanol, ethanol, ethyl ether and other polar may enhance lutein extraction efficiency (Yen et al., 2012). Macias-Sanchez et al. (2010) also showed that the extracts from Scenedesmus almeriensis by SFE had h low lutein but high b-carotene (less polar). Combined method in Ruen-Ngam et al. (2012) with organic solvent eluent pretreatment has a good performance (53% lutein recovery and 43% selectivity with lutein content of 7.9 g/kg dry weight in Chlorella vulgaris). Application of supercritical fluid extraction technique in lutein extraction may be a promising extraction over the organic extraction technique from microalgae.