In this paper, a method of microwave-assisted extraction (MAE) was for the first time used to extract total triterpenoid saponins from
Ganoderma atrum, which have been reported to have several medicinal properties and uses. The extracts were directly determined by
colorimetric method without any further treatment. Compared with shaking extraction method, heat reflux extraction, supercritical fluid
carbon dioxide extraction (SFE) and normal ultrasound-assisted extraction (UAE), MAE only need 5 min to give the highest yield of
triterpenoid saponins at 0.968%, while the other extraction methods need several hours or even more than 10 h and give lower yield.
Several factors affecting the MAE extraction rate were also discussed, such as extraction time, temperature, ratio of solvent to material
and the kind of solvents. Optimal conditions of MAE can be concluded as follows: 5 min at 90 C, the ratio of solvent to material is 25 by
using 95% ethanol as the solvent.
In this paper, a method of microwave-assisted extraction (MAE) was for the first time used to extract total triterpenoid saponins fromGanoderma atrum, which have been reported to have several medicinal properties and uses. The extracts were directly determined bycolorimetric method without any further treatment. Compared with shaking extraction method, heat reflux extraction, supercritical fluidcarbon dioxide extraction (SFE) and normal ultrasound-assisted extraction (UAE), MAE only need 5 min to give the highest yield oftriterpenoid saponins at 0.968%, while the other extraction methods need several hours or even more than 10 h and give lower yield.Several factors affecting the MAE extraction rate were also discussed, such as extraction time, temperature, ratio of solvent to materialand the kind of solvents. Optimal conditions of MAE can be concluded as follows: 5 min at 90 C, the ratio of solvent to material is 25 byusing 95% ethanol as the solvent.
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