Vacuum-packed and treated with HPP (V-P); 2) Vacuum-packed,
without HPP (V) and 3) No additional treatments, used as a Control
(C).
HPP-treated samples were previously inserted into an additional
outer bag to prevent leakages. HPP was performed in a hydrostatic
pressure equipment Stansted Fluid Power Ltd. High
Pressure Iso-Lab System model FPG9400:922 (Stansted, UK) with a
vessel of 2 l capacity and a maximum working pressure of 900 MPa.
Propylene glycol (30 ml/100 ml) inwater was used as the pressuretransmitting
medium. The conditions were 500 MPa for 5 min.
Compression rate was 5 MPa/s. The pressurization was carried out
at room temperature (20 C).
After the treatments, samples were stored at 10 C for up to 21
days. The determinations were carried out at 0, 15, 21 days of
refrigerated storage.