Ultrasound assisted extraction (USAE)
2 g of dried E. arvense herb powder was extracted with 50 mL of
applicable solvent (methanol or 80% aqueous solution of methanol)
in ultrasonic bath with temperature regulation (Bandelin Elec-
tronic, Sonorex RK 100H, Germany) at 60 ◦ C for 30 min. Extract
was filtered, and plant material was extracted afterwards with two
portions
of the solvent by the same way. Extracts were filtered,
combined, and evaporated to dryness. The residues were dissolved