Materials and Methods
2.1 Sample Source and Preparation Three different brands of seasonings were purchased from Eke-Awka Market in Awka, Southern Nigeria. They were ground into powder using a food blender and transferred into polythene bags labeled X, Y, and Z. The bags were stored in a dessicator containing calcium chloride to avoid absorption of moisture. 2.2 Proximate Analysis Proximate analyses for moisture, crude fat, crude protein, and ash were carried out in accordance with the official methods of the Association of Official Analytical Chemists (AOAC) [16]. Total carbohydrates were estimated by difference: 100- (% moisture + % crude protein + % crude fat + % ash content). 2.3 Determination of MSG content This was done by titrimetry. 0.5g of each sample was dissolved in 20ml of distilled water and four drops of bromothymol blue indicator solution were added. This was titrated with 0.1M sodium hydroxide (NaOH) to a blue end point and the titer value, V was recorded. The percentage MSG was calculated thus: % MSG = % glutamic acid x 1.15 % glutamic acid = (V x 14.71 x 100) / (weight of sample x 1000) Where 1ml of 0.1M NaOH=14.71mg of glutamic acid and 1.15 is factor of MSG. V is titer value of sample in ml (1) 2.4 Determination of mineral content 1g of sample was digested with 20ml of 2:1 HNO3/HClO4 and heated until white fumes were evolved. The digested samples were then filtered into standard 50ml volumetric flask and made up to the mark with distilled water. The minerals copper (Cu), zinc (Zn), cadmium (Cd), and lead (Pb) were determined using air acetylene flame atomic absorption spectrometry (BUCK Scientific, Model 200A). Glassware used for analyses were thoroughly cleaned and all reagents used were of analytical grade.
Materials and Methods
2.1 Sample Source and Preparation Three different brands of seasonings were purchased from Eke-Awka Market in Awka, Southern Nigeria. They were ground into powder using a food blender and transferred into polythene bags labeled X, Y, and Z. The bags were stored in a dessicator containing calcium chloride to avoid absorption of moisture. 2.2 Proximate Analysis Proximate analyses for moisture, crude fat, crude protein, and ash were carried out in accordance with the official methods of the Association of Official Analytical Chemists (AOAC) [16]. Total carbohydrates were estimated by difference: 100- (% moisture + % crude protein + % crude fat + % ash content). 2.3 Determination of MSG content This was done by titrimetry. 0.5g of each sample was dissolved in 20ml of distilled water and four drops of bromothymol blue indicator solution were added. This was titrated with 0.1M sodium hydroxide (NaOH) to a blue end point and the titer value, V was recorded. The percentage MSG was calculated thus: % MSG = % glutamic acid x 1.15 % glutamic acid = (V x 14.71 x 100) / (weight of sample x 1000) Where 1ml of 0.1M NaOH=14.71mg of glutamic acid and 1.15 is factor of MSG. V is titer value of sample in ml (1) 2.4 Determination of mineral content 1g of sample was digested with 20ml of 2:1 HNO3/HClO4 and heated until white fumes were evolved. The digested samples were then filtered into standard 50ml volumetric flask and made up to the mark with distilled water. The minerals copper (Cu), zinc (Zn), cadmium (Cd), and lead (Pb) were determined using air acetylene flame atomic absorption spectrometry (BUCK Scientific, Model 200A). Glassware used for analyses were thoroughly cleaned and all reagents used were of analytical grade.
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