Design of primers
To amplify exact DNA fragment lengths, a set of 17
oligonucleotide including 6 forward and 11 reverse
primers were designed using Oligo v.5 software,
on the basis of the known pUC57 and pET21
sequences. Thermodynamic details of primer dimers
were considered by the Oligo v.5 software. The
sequence of the primers with their characters and the
corresponding expected amplified products have been
displayed in Tables 1 and 2, respectively.