Film NANI controlled S. cerevisiae growth and inhibited the growth of L. innocua, present together in the surface of refrigerated Port Salut cheese. In the case of nisin, the support in the film determined a greater effectiveness than its direct application. Additionally, it inhibited the development of a postprocessing mixed culture (S. cerevisiae and L. innocua) contamination of a covered Port Salut cheese along a storage of 8 days at 7 ± 1 C.