1. Pump
•The role of HPLC pump is to deliver the mobile phase through the system, typically at a flow rate of 1 mL/min, in a controlled, accurate, and precise manner.
•Most commercially available HPLC pumping systems and connecting lines are made of grade ANSI 316 stainless steel, which can withstand the pressures generated and is resistant to corrosion by oxidizing agents, acids, bases, and organic solvents.
•Mineral acids and halide ions do attack stainless steel. Thus, the system should be rinsed thoroughly with water if these substances have been used in the mobile phase.