biomass, after pretreatment and cooled down, the solid
residue was separated from liquid by filter through
muslin cloth. The solid residue was then washed with
water several times. After adjusted to pH 5.0, the solid
was again separated from liquid by filtering through
muslin cloth and used as substrate for enzymatic
saccharification. While the unwashing pretreated
biomass, the sample was firstly adjusted to pH 5.0 after
pretreatment, the solid was then separated from liquid
by filtering through muslin cloth and used as substrate
for enzymatic saccharification.