For my work in Lab
I have 2 groups of samples
The first group is Viola sample, I have 45 sample, I run PCR with ITS primer
Sucsess 32 samples left 13 samples, I try to run PCR for 13 samples many time but still not good. I will reextract DNA again and for viola sample Baset give me yesterday for five sample.
The second group is Tuber sample, I have 103 sample, I run PCR with ITS1 and ITS2
many sample are good amplification but not so good when we sent to sequence
Baset told me, We can try to do with trnL primer.
For Salep project
I have 55 samples, and run metabarcoding with ITS1 and ITS2
this work is new work for me I try to make understand
Now, I'm preparing the DATA of each sample to make graph,
I make from website but I have some problem, I can't edit the text in the graph