digestion of protein. According to the values in Table 2, intestinal
CP digestibility (I_DCP), intestinal available protein
(I_ACP) and total tract available protein (T_ACP) in corn DDGS
were higher than those in barley DDGS. In situ rumen CP digestibility
(R_DCP), total CP digestibility (T_DCP: 88 vs. 90%)
and rumen available protein (R_ACP) in barley DDGS were
higher than that in corn DDGS. Compared with corn DDGS, the
R_DCP of barley DDGS was significantly higher (P < 0.05). It was
in good agreement with the above-mentioned that PC fraction
was related to the overall protein degradability ruminants in
the rumen. The results showed there are different parameters
for both rumen degradation and intestinal digestiblity, these
phenomenon may be caused by DDGS particle size and distribution.
Although particle size and distribution were not
estimated, a previous study suggested that degradability and
digestiblity parameter values could be gotten from the in situ
trial [33].
3.4. Protein molecular structure features
The absorbance peak height and area intensities of protein
amide I and amide II and protein secondary structure (in
terms of alpha-helix, beta-sheet) in corn DDGS and barley
DDGS were presented in Table 3. The amide I and amide II
structure were associated with protein molecular composition.
There were no differences between corn DDGS and
barley DDGS in amide I height (A_I_H) and amide II height
(A_II_H) and their ratio of amide I to II (R_I_II_H), even though
CP contents in these two types of DDGS were significant
differences. Corn DDGS in amide I area was extremely
(p < 0.05) higher than barley DDGS (A_I_A). It could be
affected by energy feedstock variety, heat processing conditions
and so on [2,34]. Barley DDGS showed lower (p < 0.05)
value in a-helix height (alpha_H) and b-sheet height (beta_H)
and their ratio of a-helix to b-sheet (R_a_b) than corn DDGS.
Although, these measures were statistically significant, the
digestion of protein. According to the values in Table 2, intestinal
CP digestibility (I_DCP), intestinal available protein
(I_ACP) and total tract available protein (T_ACP) in corn DDGS
were higher than those in barley DDGS. In situ rumen CP digestibility
(R_DCP), total CP digestibility (T_DCP: 88 vs. 90%)
and rumen available protein (R_ACP) in barley DDGS were
higher than that in corn DDGS. Compared with corn DDGS, the
R_DCP of barley DDGS was significantly higher (P < 0.05). It was
in good agreement with the above-mentioned that PC fraction
was related to the overall protein degradability ruminants in
the rumen. The results showed there are different parameters
for both rumen degradation and intestinal digestiblity, these
phenomenon may be caused by DDGS particle size and distribution.
Although particle size and distribution were not
estimated, a previous study suggested that degradability and
digestiblity parameter values could be gotten from the in situ
trial [33].
3.4. Protein molecular structure features
The absorbance peak height and area intensities of protein
amide I and amide II and protein secondary structure (in
terms of alpha-helix, beta-sheet) in corn DDGS and barley
DDGS were presented in Table 3. The amide I and amide II
structure were associated with protein molecular composition.
There were no differences between corn DDGS and
barley DDGS in amide I height (A_I_H) and amide II height
(A_II_H) and their ratio of amide I to II (R_I_II_H), even though
CP contents in these two types of DDGS were significant
differences. Corn DDGS in amide I area was extremely
(p < 0.05) higher than barley DDGS (A_I_A). It could be
affected by energy feedstock variety, heat processing conditions
and so on [2,34]. Barley DDGS showed lower (p < 0.05)
value in a-helix height (alpha_H) and b-sheet height (beta_H)
and their ratio of a-helix to b-sheet (R_a_b) than corn DDGS.
Although, these measures were statistically significant, the
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