2.3.7. Linearity
Equation of the regression line formula and correlation coefficient were Y= 10.0031X+ 14.8232, and r2 = 0.99908 for artemisinin. Excellent linearity was obtained for compounds between peak areas and concentrations of 0.1–100_g/ml.
2.3.8. Limits of detection and quantification
Limits of detection (LOD) were established at a signal-tonoise ratio (S/N) of 3. Limits of quantification (LOQ) were established at signal-to-noise ratio (S/N) of 10. LOD and LOQ were experimentally verified by six injections of artemisinin at the LOD and LOQ concentrations. The LOD was calculated to be 0.03; the LOQ was calculated to be 0.1_g/ml for artemisinin.
2.3.9. Statistical analysis of data
An MSTATC computer package program was used for statistical analysis according to completely randomized block design. The least significant differences among the mean values were calculated in the 5% probability levels.