Koji preparation
Sixty percent of soybean was soaked in water for 6-8 h then autoclaved at 121C for 40 min. Forty percent
of wheat bran was roasted to dark brown and broken. The 32% of total wheat bran was ground to powder and
mixed with culture before spreading to raw material to ensure mixing through of raw material. Spores of A.
oryzae S. NPUST-FS-206-A1 (0.1%) were inoculated into raw material and incubated at 30C for 72h.