4. Conclusion
TTP was used for simultaneous purification of APS and aloe protein
via a single-step extraction. Under the optimized conditions
of 26.35% (w/w) ammonium sulfate and 20.82% (w/w) t-butanol at
30 ◦C with pH 6.5, higher extraction efficiencies of APS and protein
were obtained. APS in the lower phase was further purified by dialysis
with a purity of 81.7%. The molecular weight of this obtained
protein was approximately 10–15 kDa as determined via SDS-PAGE
analysis.