Determination of antioxidant activity
The methods described below were based on procedures previ-
ously described (Chan et al., 2008).
2.6.1. DPPH radical–scavenging activity
Different dilutions of extracts (1 ml) were added to 2 ml of
2,2-diphenyl-1-picrylhydrazyl (5.9 mg/100 ml methanol). Absor-
bance was measured at 517 nm after 30 min. Radical–scavenging
ability was calculated as IC 50 and expressed as expressed as AEAC
in mg ascorbic acid/100 g as follows:
AEAC ðmg AA=100 gÞ ¼
IC 50ðascorbateÞ
IC 50ðsampleÞ
? 10 5
the IC 50 of ascorbic acid used for calculation of AEAC was
0.00387 mg/ml.