Quantification of fluorescence intensity demonstrated that antibody based CRCN protein detection was linear across this range of protein concentrations (Fig. 3A). The CRCN antibody did not recognise any proteins extracted from muscle tissue (C) as a negative control (Fig. 3A). This clearly demonstrated that CRCN protein could be quantitatively detected from a complex mixture of proteins extracted from prawn epithelial tissue. However, no significant differences were observed in the amount of CRCN protein between the 0 and 100 mg/kg Axn diet treatments, or between animals exposed to black or white substrates for 2 h (Fig. 3B). A large amount of variation (up to 3-fold difference was recorded in the abundance of CRCN protein between individuals (data not shown)