Five species of frogs from North An ~ e n c as urvive extensive freezing of their body fluids to
temperatures as low as 8 ° C for periods lasting at least 2 wk. These frogs hibernate in leaf litter where
subzero temperatures commonly occur during the winter. The onset of freezing triggers Liver
glycogenolysis and the production of high concentrations of glucose or glycerol (to 100 X normal) that
functions as a cryoprotectant against freezing Injury. Concomitantly the release of the latent heat of
crystallization as body water freezes promotes the continued function of the cardiovascular system for
many hours and serves to distribute glucose throughout the body. The water content of major organs is
reduced by 50 U/:> or more during the first 24 h of freezing, wlth the water being relocated and frozen in
other body spaces. Organ dehydration functions to concentrate cryoprotectant and to reduce mechanical
damage by ice during freezing. As freezing progresses, breathing, heart beat, and most other v~tal
functions cease, but reanimation occurs ~ v ~ t hal nf ew hours after thawing. The evolution of freeze tolerance
in these animals illustrates the h~ghlyf lexible capacities of frogs to adapt to stressful environments