and then incubated at 55 °C for 6 hours.
Subsequently, proteins were precipitated using phenol/
chloroform (1:1) once, followed by phenol/ chloroform/
isoamylalcohol (25:24:1), centrifuged at 13,000 g for 10 min (4
°C) twice, and finally washed with chloroform (1:1). The upper
aqueous layer was removed,