A principle was adopted that the dietary fat content should not exceed
4% of the dietary dry matter, hence then the addition of fat was determined after fat
content in C diet analysis. Because fat content was l.8% of the dietary dry matter, 2%
of additional fat was supplemented. The animals received 560 g fat supplement to dry
matter (280 g twice a day to concentreate feed). Refusals were recorded daily and feed
samples were collected weekly. No concentrate refusals were stated. Feed samples were
analysed according to AOAC [2005]. Animals were fed twice a day at 8.00 a.m. and
4 p.m. During the sampling period, rumen contents (200 ml) were withdrawn through
the cannulae directly before and then 3 and 6 hours after morning feeding [Grummer
et al. 1993]. Immediately after withdrawal, rumen contents were strained through four
layers of cheesecloth to obtain rumen fluid in which the following determinations were
made: pH – using a CP-1-4 type pH meter, ammonia nitrogen by the Nessler method
[Szumacher-Strabel et al. 2002], and volatile fatty acids according to Tangerman
and Nagengast [1996] using a gas chromatograph (VARIAN STAR CP 3800) with
a Nukol™ – Bonded Free Fatty Acid Phase column (30 m × 0.25 mm, df 0.25 µm,
SUPELCO)
A principle was adopted that the dietary fat content should not exceed
4% of the dietary dry matter, hence then the addition of fat was determined after fat
content in C diet analysis. Because fat content was l.8% of the dietary dry matter, 2%
of additional fat was supplemented. The animals received 560 g fat supplement to dry
matter (280 g twice a day to concentreate feed).คราวที่ถูกบันทึกไว้ทุกวัน และตัวอย่างอาหาร
รวบรวมรายสัปดาห์ ไม่มีการปฏิเสธสมาธิ เป็น ระบุ ตัวอย่างการป้อน
วิเคราะห์ตามไม่ [ 2005 ] สัตว์เป็นอาหารวันละสองครั้งในเวลา 8.00 น. และ
4 ทุ่ม ในช่วงระยะเวลาของแต่ละคน , เนื้อหา ( 200 ml ) ถูกถอนผ่าน
ตัวโดยตรงก่อนแล้ว 3 และ 6 ชั่วโมงหลังอาหารเช้า [ grummer
et al . 2536 ] Immediately after withdrawal, rumen contents were strained through four
layers of cheesecloth to obtain rumen fluid in which the following determinations were
made: pH – using a CP-1-4 type pH meter, ammonia nitrogen by the Nessler method
[Szumacher-Strabel et al. 2002], and volatile fatty acids according to Tangerman
and Nagengast [1996] using a gas chromatograph (VARIAN STAR CP 3800) with
a Nukol™ – Bonded Free Fatty Acid Phase column (30 m × 0.25 mm, df 0.25 µm,
SUPELCO)
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