The Gel Doc 2000, ChemiDoc and ChemiDoc XRS Systems are easy-to-use instruments. In the
TDS Quantity One Acquisition screen on your computer, select Live/Focus mode and adjust
your image position, size, focus, and intensity using the lens controls. After the image is
optimized, capture the image. A typical procedure is described below.
5.1.1 Switch on the Universal Hood system
1. Turn on the Universal Hood main switch (on the rear left side of the cabinet).
2. Turn on the computer and start the software.
3. Select the acquisition mode from the File menu.
5.1.2 Position your gel
1. Either open the front door or open the drawer of the Hood.
2. Center your gel on the Transilluminator platen and close the door.
3. Press the Epi-Illumination button to turn on the Epi-White lights.
4. Adjust the lens Aperture, Zoom, and Focus while looking at the computer screen.
5. Open the door and reposition the gel if necessary.
6. If using white light conversion screen or white light transillumiator, focusing is easily
achieved if the Iris is slightly closed.
5.1.3 Acquire an image
1. Press the appropriate light source for your sample
2. Select an integration time (see software manual for details).
3. When a satisfactory image is seen click Capture.
5.1.4 Acquiring an image with Flat Fielding:
NOTE: This applies to ChemiDoc XRS systems only where Flat Fielding is made available
for acquiring images with UV or white light Transillumination.
1. Center your sample on the platen.
2. Turn ON the UV/White Light Transilluminator.
3. Adjust zoom, focus and iris to get the best possible image in Live/Focus mode.
4. Make sure that the box labeled “Flat Fielding” is checked to enable Flat Fielding.
5. Select Auto Expose or Manual Expose.
6. Following the exposure a window will open asking if you would like to use a saved reference
image for Flat Fielding.
7. Select NO unless you have recently acquired a Flat Field reference image and are still using
the same Illumination and Lens settings.
8. Select YES if you are using the same lens and illumination settings to use previously
generated Flat Field reference image file for Flat Field correction.