Incubate the ligation mixture at room temperature (22°C) for 1 hour. If maximal number of
transformants is required, incubate overnight at 4°C.
Note. During the ligation prepare competent E.coli cells using the provided set of solutions for preparation
of competent cells – TransformAid Bacterial Transformation Kit. To enable blue/white screening, choose
only strains having lacZ∆M15 mutation, for example, XL1-Blue, ER1727, JM109 or other. Refer to
transformation protocols on next page.